Journal: The American Journal of Clinical Nutrition

Article Title: Vitamin E absorption and kinetics in healthy women, as modulated by food and by fat, studied using 2 deuterium-labeled α-tocopherols in a 3-phase crossover design

doi: 10.1093/ajcn/nqz172

Figure Lengend Snippet: Subject baseline characteristics, shown by intervention 1

Article Snippet: Briefly, 500 μL plasma was mixed with 50 μL HDL cholesterol precipitation reagent (Stanbio, catalog #0599–020) and the mixture kept on ice for 5 min to allow lipoprotein precipitation.

Techniques: Fat

Journal: The American Journal of Clinical Nutrition

Article Title: Vitamin E absorption and kinetics in healthy women, as modulated by food and by fat, studied using 2 deuterium-labeled α-tocopherols in a 3-phase crossover design

doi: 10.1093/ajcn/nqz172

Figure Lengend Snippet: Apolipoprotein B/E (apoB/E) precipitation and HDL isolation. HDLs were isolated from in vivo plasma samples (collected up to 20 min postinfusion) by precipitating apoE- and apoB-containing lipoproteins using magnesium/dextran sulfate (n = 10 per group, except subject 5’s sample was lost for 40% intervention due to technical difficulties). (A) ApoB/apoE precipitation, concentrations (mean ± SEM), d6-α-T half-life averaged 3.0 min; none of the curve parameters were significantly different between the 2 interventions, F-test P = 0.3826. (B) HDL, concentrations [40% fat, d6-α-T (nmol/L plasma) = 20.6X + 21.0; 0% fat, d6-α-T = 21.4X + 20.5; where X = minutes]; neither the slopes nor the y-intercepts were significantly different between the 2 interventions, F-test P = 0.9805. (C) d6-α-T enrichments in apoB/E and HDL particles. Only 2 samples were obtained for the 0% fat-fast intervention and are shown in C as a circle at 0 and 10 min that overlaps HDL data.

Article Snippet: Briefly, 500 μL plasma was mixed with 50 μL HDL cholesterol precipitation reagent (Stanbio, catalog #0599–020) and the mixture kept on ice for 5 min to allow lipoprotein precipitation.

Techniques: Isolation, In Vivo, Clinical Proteomics

Journal: Journal of the American College of Nutrition

Article Title: Diet Quality and Biomarker Profiles Related to Chronic Disease Prevention: The Multiethnic Cohort Study

doi: 10.1080/07315724.2019.1635921

Figure Lengend Snippet: Biomarker Levels by Tertiles of Dietary Indices – Lipid and Glucose Metabolism 1

Article Snippet: A Cobas Mira Plus Chemistry autoanalyzer was used to determine concentrations of CRP, total cholesterol, HDL, triglycerides (all kits from Pointe Scientific), and glucose (Randox Laboratories) [ 21 ].

Techniques: Biomarker Assay